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Approx.250 pagesApprox.250 pages
The Virology Methods Manual is a comprehensive source of methods for the study, manipulation, and detection of viruses. Edited by Brian Mahy and Hillar Kangro, this work describes the most up-to-date, definitive techniques, provided by experts in each area, and presented with easy-to-use, step-by-step protocols. This new manual will satisfy the needs of virologists and all those working with viruses who need a practical guide to methods that work! - Provides up-to-date techniques by experts worldwide - Presents common, step-by-step protocols in an attractive, easy-to-use fashion - Contains useful appendices including virus taxonomy, metabolic inhibitors, and Bio-safety in the virology laboratory
"This manual acquaints the tecnician and the laboratory officer with procedures currently in use for the diagnosis of viral diseases. It does not replace formal training in a qualified laboratory and only supplements the many standard texts now available."--Page i
Baculoviruses have proven to be the most powerful and versatile eukaryotic expression vectors available. This unique laboratory manual is designed to help both beginning and experienced researchers construct and use baculovirus vector systems. It simplifies selection of the most appropriatebaculovirus vector design for a given problem, then describes each step of the implementation process--from vector construction to large-scale protein production. The book provides an understanding of how the vectors work; a biological overview of cells, viruses, plasmids, and promoters; guidelinesfor choosing optimum vectors; protocols for growing insect cells and recombinant viruses; methods of analyzing protein products and scaling up protein production; techniques for producing proteins in insect larvae; and easy-to-use maps charting available expression vectors. This comprehensiveapproach has many benefits for researchers and students alike. It allows them to understand how and why the vector system works and offers a rapid comparison of options for choosing the right virus, plasmid or promoter for vector design and construction, with a minimum amount of lost time. Themanual is an invaluable resource for every individual engaged in the production of proteins for any purpose.
A comprehensive and updated volume for the clinical virologist. • Details laboratory procedures for detecting and handling viruses, from specimen requirements and quality assurance to virus detection and identification, from the fundamentals through the latest molecular methods. • Presents the most current knowledge on the wide range of specific viral pathogens. • Includes information on services provided by federal and state public health virology laboratories. • Provides essential information for clinicians and laboratory virologists.
Cell culture techniques allow a variety of molecular and cell biological questions to be addressed, offering physiological conditions whilst avoiding the use of laboratory animals. In addition to basic techniques, a wide range of specialised practical protocols covering the following areas are included: cell proliferation and death, in-vitro models for cell differentiation, in-vitro models for toxicology and pharmacology, industrial application of animal cell culture, genetic manipulation and analysis of human and animal cells in culture.