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Containing 57 thoroughly class-tested and easily customizable exercises,Laboratory Experiements in Microbiology: Tenth Edition provides engaging labs with instruction on performing basic microbiology techniques and applications for undergraduate students in diverse areas, including the biological sciences, the allied health sciences, agriculture, environmental science, nutrition, pharmacy, and various pre-professional programs. The Tenth Edition features an updated art program and a full-color design, integrating valuable micrographs throughout each exercise. Additionally, many of the illustrations have been re-rendered in a modern, realistic, three-dimensional style to better visually engage students. Laboratory Reports for each exercise have been enhanced with new Clinical Applications questions, as well as question relating to Hypotheses or Expected Results. Experiments have been refined throughout the manual and the Tenth Edition includes an extensively revised exercise on transformation in bacteria using pGLO to introduce students to this important technique.
This book aims to provide basic practical guidelines for microbiology and biotechnology students. All experiments have been carefully written in a clear and concise form. Major topics covered include basic microbiology practical's including antibiotic sensitivity test, Gram’s staining, Methylene Blue Reductase (MBRT) Test, Streak plate method and Food technology methods such as determination of detergent in milk, to separate the plant pigments in a food sample. This book also provides basic bioinformatics practical guidelines that can be used in dairy and food microbiology. For undergraduate (B. Sc. & B. Tech) and graduate (M. Sc.) students in various branches of biology, the book presents up-to-date fundamental information about the significant aspects of dairy microbiology as well as food microbiology. Personnel in the food industry who have little to no background in microbiology or need a refresher course in fundamental microbiological concepts and laboratory procedures will also find this book useful.
Biochemistry laboratory manual for undergraduates – an inquiry based approach by Gerczei and Pattison is the first textbook on the market that uses a highly relevant model, antibiotic resistance, to teach seminal topics of biochemistry and molecular biology while incorporating the blossoming field of bioinformatics. The novelty of this manual is the incorporation of a student-driven real real-life research project into the undergraduate curriculum. Since students test their own mutant design, even the most experienced students remain engaged with the process, while the less experienced ones get their first taste of biochemistry research. Inclusion of a research project does not entail a limitation: this manual includes all classic biochemistry techniques such as HPLC or enzyme kinetics and is complete with numerous problem sets relating to each topic.
Recent advances in imaging technology reveal, in real time and great detail, critical changes in living cells and organisms. This manual is a compendium of emerging techniques, organized into two parts: specific methods such as fluorescent labeling, and delivery and detection of labeled molecules in cells; and experimental approaches ranging from the detection of single molecules to the study of dynamic processes in organelles, organs, and whole animals. Although presented primarily as a laboratory manual, the book includes introductory and background material and could be used as a textbook in advanced courses. It also includes a DVD containing movies of living cells in action, created by investigators using the imaging techniques discussed in the book. The editors, David Spector and Robert Goldman, whose previous book was Cells: A Laboratory Manual,are highly respected investigators who have taught microscopy courses at Cold Spring Harbor Laboratory, the Marine Biology Laboratory at Woods Hole, and Northwestern University.
Concepts in Biology is a short, student-friendly text organized in a traditional manner. It has very little botany and presents a human-oriented approach to the animal unit. Professors and students appreciate the low cost of this title, and that it is written for students who are not biology majors.
Lab Manual is intended to be a handy reference for undergraduate and postgraduate students in life science and allied fields. The book covers fundamental exercises as well as advanced protocols, along with authentic explanation of various techniques and precautions pertaining to common errors in the laboratory. It is a complete instruction manual that imparts knowledge on principles, protocols and applications on techniques of biochemistry, immunology and biotechnology accurately in a user-friendly style.
Fission yeast are unicellular, rod-shaped fungi that divide by medial fission. Studies using fission yeast were instrumental in identifying fundamental mechanisms that govern cell division, differentiation, and epigenetics, to name but a few. Their rapid growth rate, genetic malleability, and similarities to more complex eukaryotes continue to make them excellent subjects for many biochemical, molecular, and cell biological studies. This laboratory manual provides an authoritative collection of core experimental procedures that underpin modern fission yeast research. The contributors describe basic methods for culturing and genetically manipulating fission yeast, synchronization strategies for probing the cell cycle, technologies for assessing proteins, metabolites, and cell wall constituents, imaging methods to visualize subcellular structures and dynamics, and protocols for investigating chromatin and nucleic acid metabolism. Modifications to techniques commonly used in related species (e.g., budding yeast) are noted, as are useful resources for fission yeast researchers, including various databases and repositories. The well-studied fission yeast Schizosaccharomyces pombe is the focus throughout, but the emerging model S. japonicus-a larger, dimorphic species with several desirable characteristics-is also covered. This manual is an important reference for existing fission yeast laboratories and will serve as an essential start-up guide for those working with fission yeast for the first time.
Laboratory Manual for Biotechnology provides students with the basic laboratory skills and knowledge to pursue a career in biotechnology. The manual, written by four biotechnology instructors with over 20 years of teaching experience, incorporates instruction, exercises, and laboratory activities that the authors have been using and perfecting for years. These exercises and activities serve to engage students and help them understand the fundamentals of working in a biotechnology laboratory. Building students' skills through an organized and systematic presentation of materials, procedures, and tasks, the manual will help students explore overarching themes that relate to all biotechnology workplaces. The fundamentals in this manual are critical to the success of research scientists, scientists who develop ideas into practical products, laboratory analysts who analyze samples in forensic, clinical, quality control, environmental, and other testing laboratories.