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Of mouse development -- Setting up a colony for the production of transgenic mice -- Recovery, culture, and transfer of embryos -- Introduction of new genetic information into the developing mouse embryo -- Iolation of pluripotential stem cell lines -- Techhniques for visualizing genes and gene products -- In vitro culture of eggs, embryos, and teratocarcinoma cels -- Chemicals, supplies, and solutions.
The success of Assisted Reproductive Technology is critically dependent upon the use of well optimized protocols, based upon sound scientific reasoning, empirical observations and evidence of clinical efficacy. Recently, the treatment of infertility has experienced a revolution, with the routine adoption of increasingly specialized molecular biological techniques and advanced methods for the manipulation of gametes and embryos. This textbook – inspired by the postgraduate degree program at the University of Oxford – guides students through the multidisciplinary syllabus essential to ART laboratory practice, from basic culture techniques and micromanipulation to laboratory management and quality assurance, and from endocrinology to molecular biology and research methods. Written for all levels of IVF practitioners, reproductive biologists and technologists involved in human reproductive science, it can be used as a reference manual for all IVF labs and as a textbook by undergraduates, advanced students, scientists and professionals involved in gamete, embryo or stem cell biology.
This intensive manual provides students with valuable information and insights into animal development at the organismal, cellular, and subcellular levels. The book uses both descriptive and investigative approaches that emphasize techniques, key experiments, and data analysis. - Provides a broad introductory view of developmental systems - Teaches both classical embryology and modern experimental approaches - Contains seventeen laboratory exercises, written in step-by-step style - Organized with additional notes to students and preparators - Lists questions and references for each exercise - Special chapters give introductions to the scientific process, use of the microscope, and the writing of scientific papers - Illustrated with detailed line drawings
Provides background information and detailed protocols for developing a mouse colony and using the animals in transgenic and gene-targeting experiments. The protocols list the animals, equipment, and reagents required and step-by-step procedures. Topics include in vitro culture of preimplantation embryos, surgical procedures, the production of chimeras, and the analysis of genome alterations. The third edition adds protocols for cloning mice, modifying embryonic stem cells, intracytoplasmic sperm injection, and cryopreservation of embryos.
The generation of mutant mice raises many questions about the best means of phenotypic analysis, breeding, and maintenance. The answers are now available from two experts with a wealth of detailed knowledge never previously assembled in one volume. Informal and highly practical, this handbook provides step–by–step methods for troubleshooting experiments, from the basics of gene targeting through the analysis of postnatal effects.
This is a lab manual for a college-level human anatomy course. Mastery of anatomy requires a fair amount of memorization and recall skills. The activities in this manual encourage students to engage with new vocabulary in many ways, including grouping key terms, matching terms to structures, recalling definitions, and written exercises. Most of the activities in this manual utilize anatomical models, and several dissections of animal tissues and histological examinations are also included. Each unit includes both pre- and post-lab questions and six lab exercises designed for a classroom where students move from station to station. The vocabulary terms used in each unit are listed at the end of the manual and serve as a checklist for practicals.