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So much has been learned about RNA in the past ten years that the ability to purify, analyze, and manipulate RNA molecules is now essential in all kinds of bioscience. Initiating RNA research can be intimidating but the new book RNA: A Laboratory Manualprovides a broad range of up-to-date techniques presented in a functional framework, so that any investigator can confidently handle RNA and carry out meaningful experiments, from the most basic to the highly sophisticated. Originating in three of the field's most prominent laboratories, this manual provides the necessary background and strategies for approaching any RNA investigation, as well as detailed protocols and extensive tips and troubleshooting information. It is required reading for every research laboratory in the life sciences.
Recent advances in imaging technology reveal, in real time and great detail, critical changes in living cells and organisms. This manual is a compendium of emerging techniques, organized into two parts: specific methods such as fluorescent labeling, and delivery and detection of labeled molecules in cells; and experimental approaches ranging from the detection of single molecules to the study of dynamic processes in organelles, organs, and whole animals. Although presented primarily as a laboratory manual, the book includes introductory and background material and could be used as a textbook in advanced courses. It also includes a DVD containing movies of living cells in action, created by investigators using the imaging techniques discussed in the book. The editors, David Spector and Robert Goldman, whose previous book was Cells: A Laboratory Manual,are highly respected investigators who have taught microscopy courses at Cold Spring Harbor Laboratory, the Marine Biology Laboratory at Woods Hole, and Northwestern University.
Introduction to immunochemistry for molecular biologists and other nonspecialists. Spiral.
Praise for Allen & Harper's "Laboratory Manual for Anatomy and Physiology " Connie Allen and Valerie Harper... have done an excellent job of preparing an A & P lab manual that students will appreciate and instructors will find easy to teach." "- - Moges Bizuneh, Ivy Tech State College - Indianapolis " I am very impressed with the quality and the readability of this lab manual." "- - Karen K. McLellan, Indiana University-Purdue University Fort Wayne " ... .this lab manual is an excellent one. It is well-written, has just the right amount of written text, and contains very good illustrations, photos, lab activities and questions." "- - Janet Lichti, Ivy Tech State College - Lafayette Also available PowerAnatomy, An Online Laboratory Manual Connie Allen, Valeria Harper, Susan Baxley ISBN: 0-471-44558-4 "PowerAnatomy combines over 100 of Primal's exquisitely detailed, 3D models of the human body, along with text, exercises, and review materials. Fetal Pig Dissection: A Laboratory Guide, 2nd Edition Connie Allen and Valerie Harper ISBN: 0-471-70138-6, Paper Cat Dissection: A Laboratory Guide, 2nd Edition Connie Allen and Valerie Harper ISBN: 0-471-70141-6, Paper
A Laboratory Manual for Forensic Anthropology approaches forensic anthropology as a modern and well-developed science, and includes consideration of forensic anthropology within the broader forensic science community, with extensive use of case studies and recent research, technology and challenges that are applied in field and lab contexts. This book covers all practical aspects of forensic anthropology, from field recoveries, to lab analyses, emphasizing hands-on activities. Topics include human osteology and odontology, examination methods, medicolegal significance, scene processing methods, forensic taphonomy, skeletal processing and sampling, sex estimation, ancestry estimation, age estimation, stature estimation, skeletal variation, trauma analysis, and personal identification. Although some aspects are specific to the United States, the vast majority of the material is internationally-relevant and therefore suitable for forensic anthropology courses in other countries. Provides a comprehensive lab manual that is applicable to coursework in forensic anthropology and archaeology Covers all practical aspects of forensic anthropology, from field recoveries, to lab analyses Includes discussions of human osteology and odontology, examination methods, medicolegal significance, scene processing methods, forensic taphonomy, skeletal processing and sampling, sex estimation, and more Emphasizes best practices in the field, providing an approach that is in line with today's professional forensic anthropology
Synthetic Biology: A Lab Manual is the first manual for laboratory work in the new and rapidly expanding field of synthetic biology. Aimed at non-specialists, it details protocols central to synthetic biology in both education and research. In addition, it provides all the information that teachers and students from high schools and tertiary institutions need for a colorful lab course in bacterial synthetic biology using chromoproteins and designer antisense RNAs. As a bonus, practical material is provided for students of the annual international Genetically Engineered Machine (iGEM) competition. The manual is based upon a highly successful course at Sweden's Uppsala University and is coauthored by one of the pioneers of synthetic biology and two bioengineering postgraduate students.An inspiring foreword is written by another pioneer in the field, Harvard's George Church: “Synthetic biology is to early recombinant DNA as a genome is to a gene. Is there anything that SynBio will not impact? There was no doubt that the field of SynBio needed ‘A Lab Manual’ such as the one that you now hold in your hands.”
This second edition laboratory manual was written to accompany Food Analysis, Fourth Edition, ISBN 978-1-4419-1477-4, by the same author. The 21 laboratory exercises in the manual cover 20 of the 32 chapters in the textbook. Many of the laboratory exercises have multiple sections to cover several methods of analysis for a particular food component of characteristic. Most of the laboratory exercises include the following: introduction, reading assignment, objective, principle of method, chemicals, reagents, precautions and waste disposal, supplies, equipment, procedure, data and calculations, questions, and references. This laboratory manual is ideal for the laboratory portion of undergraduate courses in food analysis.
This second edition of the now-classic lab manual Antibodies, by Harlow and Lane, has been revised, extended, and updated by Edward Greenfield of the Dana-Farber Cancer Center, with contributions from other leaders in the field. Once again, the manual is an essential resource for molecular biology, immunology, and cell culture labs on all matters relating to antibodies. The chapters on hybridomas and monoclonal antibodies have been recast with extensive new information and there are additional chapters on characterizing antibodies, antibody engineering, and flow cytometry. As in the original book, the emphasis in this second edition is on providing clear and authoritative protocols with sufficient background information and troubleshooting advice for the novice as well as the experienced investigator.
CRISPR/Cas-based techniques are revolutionizing the way geneticists and molecular biologists modify DNA sequences and modulate gene expression in cells and organisms. This laboratory manual presents step-by-step protocols for applying this cutting-edge technology to any system of interest. Contributors describe approaches for de.
Imaging: A Laboratory Manual is organized into three sections, each containing background information and step-by-step protocols