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HPLC has largely contributed to the development of pharmacology, biology, food research and the biomedical sciences, as demonstrated by the growing number of meetings dedicated to this topic and by the proliferation of companies offering equipment, products or services for HPLC users. It is becoming highly difficult to follow the current literature, particularly in the area of applications.
A practical introduction to the principles and methods of high-performance liquid chromatography. Classroom-tested, reflecting many new details and recent improvements in HPLC methods. Provides enough theoretical background to apply this separation method intelligently, and provides up-to-date instruction on current chromatographic practice. Covers preparation of equipment, details of techniques, and commercially available stationary phases.
Chromatography has become the most widely used technique for separation and analyses. Because a great deal of information is available on the three main types--gas chromatography (GC), liquid chromatography (LC), and thin layer chromatography (TLC)--they are usually treated separately despite their common theoretical base. This comprehensive work presents a unified study of chromatography which introduces the principles shared by each method while emphasizing the similarities and differences between the three types. The chapters covering these methods build on the introductory coverage of common principles, providing an effective overview of the subject for novices and allowing practitioners to more easily switch from one technique to another. In addition, this approach permits the use of one set of terms and symbols, making learning easier. Includes several practical examples the ways in which GC, LC, and TLC operate, as well as a section on special techniques such as chiral separations and derivatization.