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Histological observations of human embryos hitherto have been carried out using paraffin sections of 5 to 10 μm thickness, stained with the H-E method or simply with carmine. Because of the thickness, cells are arranged in 2 or 3 layers in one section and the histological details are not always clear. This book provides detailed morphological features of a very well preserved human embryo with fifteen somites. The sections are about 0.75 μm thick and stained with toluidine blue. The thinness of sections and clearness of staining reveal the histological details of this embryo very accurately. A complete set of high quality photomicrographs are presented for each of the selected sections. The high resolution of the photomicrographs will enable easy comparison with the literature. The clear presentation in this book of embryonic development is increasingly important and highly relevant for in-vitro fertilization, and thus of interest to reproductive biologists as well as anatomists.
Building the Most Complex Structure on Earth provides readers with a basic biological education an easy and understandable introduction into a new epigenetic theory of development and evolution. This is a novel theory that describes the epigenetic mechanisms of the development and evolution of animals and explains the colossal evolution and diversification of animals from a new post-genetic perspective. Modern biology has demonstrated the existence of a common genetic toolkit in the animal kingdom, but neither the number of genes nor the evolution of new genes is responsible for the development and evolution of animals. The failure to understand how the same genetic toolkit is used to produce millions of widely different animal forms remains a perplexing conundrum in modern biology. The novel theory shows that the development and evolution of the animal kingdom are functions of epigenetic mechanisms, which are the competent users of the genetic toolkit. - Provides a comprehensive view of the epigenetic aspects of reproduction, development, and evolution. - Highly rigorous, but simple enough for readers with only a basic knowledge of biology.
The success of Assisted Reproductive Technology is critically dependent upon the use of well optimized protocols, based upon sound scientific reasoning, empirical observations and evidence of clinical efficacy. Recently, the treatment of infertility has experienced a revolution, with the routine adoption of increasingly specialized molecular biological techniques and advanced methods for the manipulation of gametes and embryos. This textbook – inspired by the postgraduate degree program at the University of Oxford – guides students through the multidisciplinary syllabus essential to ART laboratory practice, from basic culture techniques and micromanipulation to laboratory management and quality assurance, and from endocrinology to molecular biology and research methods. Written for all levels of IVF practitioners, reproductive biologists and technologists involved in human reproductive science, it can be used as a reference manual for all IVF labs and as a textbook by undergraduates, advanced students, scientists and professionals involved in gamete, embryo or stem cell biology.
Somite development is a unique and important topic; unique because somitic mesoderm is the first tissue to become segmented, important because the somites - once established - exert a profound influence on other seg mental structures which form later. Somite development is of interest at a number of levels. In one aspect, demarcation of a specific number of somites and size regulation, it is a particularly intriguing example of embryonic pattern formation, 'especially in vew of its possible relation to homoeobox-controlled segmentation in insects. At another level, somite development has long been studied by compara tive anatomists, but only recently has new light been thrown on such sub jects as segmentation of the head, proposed in the 18th century by Goethe and now a live issue for developmental biologists. Somit es are simple when they first appear, but very complex structures arise from them. These include the vertebrae and the axial and other muscles and consequently there is a wealth of morphogenetic problems to be explored. Sometimes their morphogenesis is disturbed, by genetic or environ mental factors, and there are many clinical conditions which arise as a result.
Bones and Cartilage provides the most in-depth review and synthesis assembled on the topic, across all vertebrates. It examines the function, development and evolution of bone and cartilage as tissues, organs and skeletal systems. It describes how bone and cartilage develop in embryos and are maintained in adults, how bone is repaired when we break a leg, or regenerates when a newt grows a new limb, or a lizard a new tail. The second edition of Bones and Cartilage includes the most recent knowledge of molecular, cellular, developmental and evolutionary processes, which are integrated to outline a unified discipline of developmental and evolutionary skeletal biology. Additionally, coverage includes how the molecular and cellular aspects of bones and cartilage differ in different skeletal systems and across species, along with the latest studies and hypotheses of relationships between skeletal cells and the most recent information on coupling between osteocytes and osteoclasts All chapters have been revised and updated to include the latest research. - Offers complete coverage of every aspect of bone and cartilage, with updated references and extensive illustrations - Integrates development and evolution of the skeleton, as well a synthesis of differentiation, growth and patterning - Treats all levels from molecular to clinical, embryos to evolution, and covers all vertebrates as well as invertebrate cartilages - Includes new chapters on evolutionary skeletal biology that highlight normal variation and variability, and variation outside the norm (neomorphs, atavisms) - Updates hypotheses on the origination of cartilage using new phylogenetic, cellular and genetic data - Covers stem cells in embryos and adults, including mesenchymal stem cells and their use in genetic engineering of cartilage, and the concept of the stem cell niche
This is the first and only book, so far, to deal with the causal basis of evolution from an epigenetic view. By revealing the epigenetic "user" of the "genetic toolkit", this book demonstrates the primacy of epigenetic mechanisms and epigenetic information in generating evolutionary novelties. The author convincingly supports his theory with a host of examples from the most varied fields of biology, by emphasizing changes in developmental pathways as the basic source of evolutionary change in metazoans. - Original and thought provoking--a radically new theory that overcomes the present difficulties of the theory of evolution - Is the first and only theory that uses epigenetic mechanisms and principles for explaining evolution of metazoans - Takes an integrative approach and shows a wide range of learning
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"This book provides a complete look at neonatal healthcare delivery. This edition includes discussions of contemporary topics of interest, such as informatics, genetics, global health, and family-centered care, which are vital to providers caring for neonates today. The case studies and the evidence-based practice dialogues at the end of each chapter provide great opportunities for further reflection. The book is useful to a wide audience in nursing, including undergraduate and graduate nursing students, practicing neonatal and pediatric nurses, and advanced practice nurses who care for neonates." Score: 92, 4 Stars.--Doody's Medical Reviews This 'classic' has been thoroughly updated to incorporate the most up-to-date research findings and strategies for providing cost-effective and evidence-based care. New chapters address emerging infections, the late preterm infant, and neonatal care from a global perspective. Included are updated neonatal care protocols and procedures, neuroprotective risk factors, new treatments, and new trends in developmental care. Text integrates the Institute of Medicine's (10M) five competencies, reflects the Affordable Healthcare Act and the Robert Wood Johnson and 10M report "The Future of Nursing." The text continues to provide neonatal care from a physiologic and pathophysiologic approach, with a major emphasis on nursing management at the bedside and advanced practice level. Each neonatal body system is presented, along with E-B interventions to assist in understanding the 'why' behind what is seen in the clinical area. Integrative management is threaded through the text along with extensive research findings to support practice strategies and rationales for sound clinical decision-making. Topics of recent interest include iatrogenic complications, neonatal pain, use of computers or other technology in neonatal care, and neonatal AIDS. Case studies enhance understanding of both common and rare neonatal conditions. New to the Fifth Edition: New chapters: emerging infections, the late preterm infant, and neonatal care from a global perspective Updated neonatal care protocols and procedures, neuroprotective factors, new treatment modalities and new trends in developmental care Tackles the UN Millennium Development Goals (MDGs) Addresses the expansion of the nurse's role in the US and worldwide Provides case studies that lead the reader through the identification, diagnosis, treatment, and evaluation of common and rare neonatal conditions